WebJun 15, 2012 · Phosphopeptide chip technology and antibody profile. Peptide chips containing three identical arrays of 6400 phosphopeptide spots were incubated with the indicated anti-phosphotyrosine antibodies (4G10, p-TYR-100 and pY20) and a fluorescently labelled secondary antibody.
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WebApr 17, 2007 · Bound proteins were eluted with 500 mmol/L Imidazole and fractionated through 12% SDS-PAGE before their electrophoretic transfer to nitrocellulose membranes. Immobilized proteins were probed sequentially with antibodies against phosphotyrosine (4G10 antibodies; Upstate, Charlottesville, VA) followed by those against GST (Invitrogen, … WebSen Chen's publication in Sci Signal. ...against pTyr (4G10) and tubulin were from Millipore, and antibody against pMET (Tyr1234) was from GeneTex. Bmxtides were from …
Web05-1050X Sigma-Aldrich Anti-Phosphotyrosine Antibody, 4G10® Platinum Download Zoom Anti-Phosphotyrosine Antibody, 4G10 Platinum is an antibody against Phosphotyrosine for use in WB, IF, FC, IH, IP, IC, ELISA. MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. MSDS 分析证书 参考 数据表 WebAnti-Phosphotyrosine Antibody, clone 4G10® Anti-Phosphotyrosine Antibody, clone 4G10 detects tyrosine phosphorylated proteins in all species. This unique monoclonal antibody …
WebApr 12, 2024 · To measure the protein phosphotyrosine and acrosine availability, cell aliquots were fixed, permeabilized and blocked as described above (section actin polymerization). Subsequently, the samples were incubated for 1 h with specific anti-phosphotyrosine (4G10 Platinum, Sigma‒Aldrich) or anti-acrosine (ACR-2, Exbio, Praha, … WebMar 23, 2024 · The membranes were afterward incubated with mouse anti-phosphotyrosine antibody (clone 4G10; Millipore, MA, USA) diluted 1:1,000 in PBS or mouse anti-ubiquitin antibody (FK2, 1:250 dilution, monoclonal mouse antibody recognizing mono- and polyubiquitinated conjugates; ENZO Life Sciences, NY, USA) at 4°C overnight. After …
WebJun 15, 2012 · The 4G10, pY20 and p-TYR-100 antibody specificity: profiling by peptide microarrays The reversible phosphorylation of tyrosine residues is one of the most …
WebTheir tyrosine phosphorylation was examined after immunoprecipitation by western blotting using an anti-pCrk (Y 221 ) or anti-phosphotyrosine (4G10) antibody. Equal loading was checked after reprobing the membranes with an anti-CrkII (Crk) and anti-CrkL antibody. (D) Densitometric analysis of 3 independent experiments from Figures B & C is shown. bind benefits insurance timely filing limitWebMillipore’s phosphotyrosine antibodies, including the popular 4G10 clone, demonstrate sensitivity and detect multiple tyrosine phosphorylations on numerous substrates. See below for pTyr antibodies. - Find MSDS or SDS, a COA, data sheets and more information. bind benefits uhc phone numberWebAnti-Phosphotyrosine Antibody, clone 4G10 detects tyrosine phosphorylated proteins in all species. This unique monoclonal antibody is validated for use in IC, IH, IP, WB and is … bind benefits surestWebPhospho-Tyrosine (4G10) Mouse mAb recognizes a broad range of tyrosine-phosphorylated proteins and peptides. This antibody does not cross-react with proteins or peptides … bind betaflight cliWeb16-316 Sigma-Aldrich Anti-Phosphotyrosine Antibody, 4G10® Platinum, HRP Conjugate Download Zoom Detect Phosphotyrosine with Anti-Phosphotyrosine Antibody, 4G10 Platinum, HRP Conjugate (Mouse Monoclonal Antibody), … cystease apotekWeb11 rows · 4G10 Platinum, Anti-Phosphotyrosine (mouse monoclonal … bind benefits timely filingWebPhosphotyrosine-containing proteins were recovered with 4G10-agarose, and western blotted with anti-Themis2 antibodies. Panels b,c . Anti-Flag immunoprecipitates of LPS-stimulated RAW cells stably expressing Flag-tagged Themis2 were western blotted with anti-Flag-HRP and anti-Lyn kinase. bind b get_away_from_keyboard