Phis2 酵母单杂

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August undefined, 2024

Webb26 maj 2024 · 载体描述. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ One- Hybrid Library Construction & Screening Kit (#K1617-1). It contains a HIS3 nutritional reporter gene, located downstream of a … Webb实验原理: 真核生物基因的转录起始需转录因子参与，转录因子通常由DNA结合结构域 (ONA- binding domain, BD)和转录激活结构域 (acivationdomain, AD)组成.用于酵母单杂交系统的 …

pHIS2说明书-分析方法-生物在线 Lab-on-Web - BIOON

Webb人机验证 Webb9 juli 2024 · 幼儿/小学教育 -- 教育管理. 文档标签：. phis2酵母表达载体说明. 系统标签：. 酵母表达载体多克隆位点载体克隆方法说明拷贝. pHis2编号载体名称北京华越洋生物VECT2110pHis2pHis2载体基本信息出品公司:Clontech载体名称:pHis2质粒类型:酵母单杂交载体高拷贝/低拷贝 ... the railway hotel west wickham

PT3705-5 (PR32049) pHIS2

WebbpHIS2 Vector Information PT3705-5 Sold as part of Catalog #K1617-1 Restriction Map and Multiple Cloning Site (MCS) of pHIS2 Vector. Unique restriction sites are in bold. *Note: The Sac II site is resistant to cleavage. Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding ... Webb1 mars 2024 · VvACTIN or AtACTIN2 was used as internal references for grape and Arabidopsis. The qRT-PCR primers are listed in Table 1. Table 1. Gene-specific primers used for quantitative real-time PCR assays. 2.7. Transformation of Arabidopsis The 35S: VvWRKY30 vector was created by cloning genes into the pSuper1300 binary vector with … Webb6 sep. 2024 · 摘要：本文针对酵母单杂技术的基本原理、基础知识、主要用途做了简要介绍；并以Y1Hgold（pAbAi-bait）和Y187（pHis2-bait）为例，介绍了自激活检测的目的以 … the railway hotel queenstown

Phis2 酵母单杂

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http://www.coolaber.com/usr/2160/File/F/F4671.pdf Webb1、文库质量评估：理论+涂板评价交付材料：反馈质量评估结果 2、载体构建：基因合成诱饵基因构建Phis2载体交付材料：含诱饵基因的质粒 3、启动子背景筛查：Binding seq基因启动子的背景筛查交付材料：阳性克隆测序结果 word项目报告 4、文库筛选：诱饵质粒转化和文库质粒转化酵母筛选和3AT条件优化筛选结果测序交付材料：阳性克隆测序结果 …

WebbpHis2 价格： 1000元联系方式： I47-825O-882O 立刻购买买家导航载体基本信息载体质粒图谱和多克隆位点信息载体简介载体序列 LOCUS pHIS-2 7207 bp DNA SYN DEFINITION pHIS-2 ACCESSION KEYWORDS SOURCE ORGANISM … Webb质粒名称：pHis2 出品公司：Clontech 质粒类型：酵母单杂交系统克隆方法：多克隆位点，限制性内切酶载体大小： 7207bp 原核抗性： Kanamycin (卡那霉素) 真核抗性：TRP1 (HIS3 selection) 备注： Clone in cis-acting sequences for use with 1-hybrid system 载体介绍 pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify …

WebbpHIS2 PVT4037 2ug Lot no 1901093 Store at -20℃ Expiry date June.2024 pHIS2 Information Promoters: TRP1, T7, T3 Replicon: pUC ori, FI ori Plasmid classification: yeast series, yeast one hybrid vector Plasmid size: 7207bp … Webb还有一点，我看了pHIS2的序列，在HIS3前面，Pmin区域里也有我的转录因子结合位点，会不会是我的转录因子结合到那里去了？不知道有没有这个可能性啊？这个minimum …

Webb1 dec. 2024 · AD/pATM1-pHIS2 and AD-ATM1/pATM1-pHIS2 were cotransformed into the Y187 strain and cultured on SD/-Trp/-Leu for 2–3 days at 30 °C. Then, healthy and consistent clones were diluted, ...

Webb产品名称：pHIS2酵母单杂交质粒. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was … signs and symptoms of panhypopituitarismWebb16 mars 2024 · 产品说明：. Y187-pHis2 Yeast One-Hybrid Media Kit（酵母单杂培养基套装YM1010），含有用于Y187-pHis2系统酵母单杂交文库筛选的全套培养基，性价比更高 … signs and symptoms of pelvic inflammatoryhttp://muchong.com/t-9775583-1 signs and symptoms of penile cancerWebb19 nov. 2012 · 是选取atg上游2000的片段，然后整个连到phis2载体上吗？我之前做了直接取ATG上游1.5k构建到phis2上，感觉不合适。最好还是找到自己转录因子的结合motif然后取motif和其上下游几十bp的片段，然后串联3个片段构建载体比较合适。 the railway inn aldeburgh suffolkhttp://muchong.com/t-9775583-1-pid-2 signs and symptoms of period comingWebb8 juli 2024 · pHis2 编号载体名称北京华越洋生物VECT2110 pHis2 pHis2 载体基本信息出品公司: Clontech 载体名称: pHis2 质粒类型: 酵母单杂交载体高拷贝/低拷贝: -- 启动子: -- 克隆方法: 多克隆位点，限制性内切酶载体大小: 7207bp 5' 测序引物及序列: -- 3' 测序引物及序列: -- 载体标签 ... the railway inn burgess hillWebb1、pHis2-Bait或pAbAi-Bait和AD-prey质粒 2、或者Bait和Prey序列，质粒由我们构建 1、提供详细的、完整的实验报告，清晰的实验图片 2、在受体菌的阳性菌自激活和毒性验证: … the railway inn buxton